Rat Transforming Growth Factor β1 ELISA kit (E02T0009) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Transforming Growth Factor β1 ELISA kit (E02T0009)

E02T0009 Rat Transforming Growth Factor β1 ELISA kit

The Rat Transforming Growth Factor β1 ELISA kit can be used to identify samples from the rat species. Transforming Growth Factor β1 can also be called CED, DPD1, TGF beta 1, TGF beta, TGF beta 1 protein, TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGF-beta1, TGFB, Tgfb-1, tgfb1, TGFbeta, TGFbeta1, Transforming Growth Factor b1, Transforming Growth Factor beta 1, Transforming growth factor beta 1a, transforming growth factor beta-1, transforming growth factor, beta 1.

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Specifications of Rat Transforming Growth Factor β1 ELISA kit

Product Information
Cat. No.	E02T0009
Product Name	Rat Transforming Growth Factor β1 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

TGFβ1 ELISA kit uses an anti-TGFβ1 antibody and an TGFβ1-HRP conjugate in a competitive enzyme immunoassay method. TGFβ1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGFβ1 antibody binding site between TGFβ1 from samples and TGFβ1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGFβ1. Since the number of sites is limited, as more sites are occupied by TGFβ1 from the sample, fewer sites are left to bind TGFβ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFβ1 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Transforming Growth Factor β1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between TGFβ1 and analogues was observed.

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Summary of the Assay Procedure for Rat Transforming Growth Factor β1 ELISA kit

Citations of Rat Transforming Growth Factor β1 ELISA kit

E02T0009 has been referenced in the below publications：

Nonalcoholic fatty liver tissue of hepatitis TNFΑ, TGFΒ1, LEPTIN and MAPK expression and drug intervention.

THE STUDY OF PREVENTIVE AND THERAPEUTIC EFFECTS OF TRIMETAZIDINE HYDROCHLORIDE ON RAT RENAL INTERSTITIAL FIBROSIS.

Identification of Chronic Atrophic Gastritis Model Rats Differentially Expressed Proteins in Gastric Mucosa by Quantitative Proteomics and Research for Effect Targets of Chinese Medicine.

Discussion of changes of TGF-β 1，Smad-4，α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the“liver and kidney share the same origin”.

Study of the Effects of Berberine Hydrochloride on Vascular Smooth Muscle Cells in the Type 2 Diabetes Mellitus.

The renal damage caused by advanced glycation end products relating to activation of Na＋ /H ＋exchanger 1.