E02T0491 Rat Troponin I, cardiac muscle ELISA kit
The Rat Troponin I, cardiac muscle ELISA kit can be used to identify samples from the rat species. Troponin I, cardiac muscle can also be called TNNI3, CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I3, cardiac type.
Product Information | |
Cat. No. | E02T0491 |
Product Name | Rat Troponin I, cardiac muscle ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNNI3 ELISA kit uses an anti-TNNI3 antibody and an TNNI3-HRP conjugate in a competitive enzyme immunoassay method. TNNI3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNNI3 antibody binding site between TNNI3 from samples and TNNI3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNNI3. Since the number of sites is limited, as more sites are occupied by TNNI3 from the sample, fewer sites are left to bind TNNI3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNNI3 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNNI3 and analogues was observed. | |
E02T0491 has been referenced in the below publications：
Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage.
Protective effect of different doses of anidoamine against myocardial myocardial ischemia/reperfusion injury and mechanism mediated by mitochondrial ATP-sensitive potassium channel.
Protective Effect of Bisoprolol Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats by the Medium of PI3K/AKT/GS3K-b.
Experimental Study On Cardioprotection and Mechanism Of Combined Limb Remote Ischemic Postconditioning and Sevoflurane postcondmoning Against Rat Myocardial Ischemia-Reperfusion Injury and Its mechanism.
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