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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Urine protein ELISA (E02U0025)

E02U0025 Rat Urine protein ELISA

The Rat Urine protein ELISA can be used to identify samples from the rat species. Urine protein can also be called UP

Products

Specifications of Rat Urine protein ELISA

Product Information

Cat. No.

E02U0025

Product Name

Rat Urine protein ELISA

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 mg/ml

Sensitivity

0.1 mg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/mL

1 vial

STANDARD B (0.5mL)

0.5 mg/mL

1 vial

STANDARD C (0.5mL)

1.0 mg/mL

1 vial

STANDARD D (0.5mL)

2.5 mg/mL

1 vial

STANDARD E (0.5mL)

5.0 mg/mL

1 vial

STANDARD F (0.5mL)

10 mg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

UP ELISA kit uses an anti-UP antibody and an UP-HRP conjugate in a competitive enzyme immunoassay method. UP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-UP antibody binding site between UP from samples and UP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of UP. Since the number of sites is limited, as more sites are occupied by UP from the sample, fewer sites are left to bind UP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Urine protein ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between UP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Urine protein ELISA

Summary of the Assay Procedure for Rat Urine protein ELISA

Citations of Rat Urine protein ELISA

E02U0025 has been referenced in the below publications:

Effect of Huayu Tongluo Traditional Chinese Medicine on Skeleton Protein in Podocytes of Rats with Diabetic Nephropathy.Effect of Combined Prescription and Separate Prescription of Huayu Tongluo Traditional Chinese Medicine on Blood Correlation Index and the Expression of ZO-1 in Renal Podocytes of DN Rats.

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