E02V0002 Rat Vascular Cell Adhesion Molecule 1 ELISA kit
The Rat Vascular Cell Adhesion Molecule 1 ELISA kit can be used to identify samples from the rat species. Vascular Cell Adhesion Molecule 1 can also be called CD 106, CD106, CD106 Antigen, INCAM 100, L1CAM, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, INCAM-100, V-CAM 1
Product Information | |
Cat. No. | E02V0002 |
Product Name | Rat Vascular Cell Adhesion Molecule 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VCAM 1 ELISA kit uses an anti-VCAM 1 antibody and an VCAM 1-HRP conjugate in a competitive enzyme immunoassay method. VCAM 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VCAM 1 antibody binding site between VCAM 1 from samples and VCAM 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VCAM 1. Since the number of sites is limited, as more sites are occupied by VCAM 1 from the sample, fewer sites are left to bind VCAM 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VCAM 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VCAM 1 and analogues was observed. | |
E02V0002 has been referenced in the below publications：
Damage effects of high iodine on endothelial cells from the rat aorta segments.
Effect of Excessive Potassium Iodide on Rat Aorta Endothelial Cells.
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