E02V0010 Rat Vascular Endothelial Growth Factor ELISA kit
The Rat Vascular Endothelial Growth Factor ELISA kit can be used to identify samples from the rat species. Vascular Endothelial Growth Factor can also be called Vascuoar endothelial growth factor A, VEGF A, vascular endothelial growth factor A isoform 2 precursor, Vegf, VEGFA; MVCD1, VEGF, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF A Precursor.
Product Information | |
Cat. No. | E02V0010 |
Product Name | Rat Vascular Endothelial Growth Factor ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VEGF ELISA kit uses an anti-VEGF antibody and an VEGF-HRP conjugate in a competitive enzyme immunoassay method. VEGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VEGF antibody binding site between VEGF from samples and VEGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VEGF. Since the number of sites is limited, as more sites are occupied by VEGF from the sample, fewer sites are left to bind VEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VEGF and analogues was observed. | |
E02V0010 has been referenced in the below publications：
Protective effects of mci-186 on transplantation of bone marrow stromal cells in rat ischemic stroke model.
Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.
Effects of ischemic preconditioning on expression of VEGF, GLUT1 and BAI1 in focal cerebral infarction rats.
Effects of alcohol extract from root of wintersweet on expression of HIF-1α,VEGF and MMP-3 in adjuvant arthritis rats.
Effect of edaravone on regeneration of neurons in rats after cerebral ischemia/reperfusion injury.
Hypoxic Preconditioning Augments the Therapeutic Efficacy of Bone Marrow Stromal Cells in a Rat Ischemic Stroke Model.
Visualized identification of the maximal surgical delay effect in a rat flap model.
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