E02V0037 Rat Vasoactive intestinal peptide ELISA kit
The Rat Vasoactive intestinal peptide ELISA kit can be used to identify samples from the rat species. Vasoactive intestinal peptide can also be called VIP, vasoactive intestinal peptide, PHM27.
Specifications of Rat Vasoactive intestinal peptide ELISA kit
Product Information | |
Cat. No. | E02V0037 |
Product Name | Rat Vasoactive intestinal peptide ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VIP ELISA kit uses an anti-VIP antibody and an VIP-HRP conjugate in a competitive enzyme immunoassay method. VIP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VIP antibody binding site between VIP from samples and VIP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VIP. Since the number of sites is limited, as more sites are occupied by VIP from the sample, fewer sites are left to bind VIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VIP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Vasoactive intestinal peptide ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VIP and analogues was observed. | |
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Summary of the Assay Procedure for Rat Vasoactive intestinal peptide ELISA kit
Citations of Rat Vasoactive intestinal peptide ELISA kit
E02V0037 has been referenced in the below publications：
Effects of KuiKeLing and Its Modified Formula on Bowel Movement and Gastrointestinal Peptide Hormones in Colitis Model Rats
