E02V0006 Rat Very Low Density Lipoproteins ELISA kit
The Rat Very Low Density Lipoproteins ELISA kit can be used to identify samples from the rat species. Very Low Density Lipoproteins can also be called Pre-β-Lipoprotein, Pre-Beta Lipoprotein, VLDL.
Product Information | |
Cat. No. | E02V0006 |
Product Name | Rat Very Low Density Lipoproteins ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 mg/ml |
Sensitivity | 1.0 mg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/ml | 1 vial |
STANDARD B (0.5mL) | 50 mg/ml | 1 vial |
STANDARD C (0.5mL) | 100 mg/ml | 1 vial |
STANDARD D (0.5mL) | 250 mg/ml | 1 vial |
STANDARD E (0.5mL) | 500 mg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 mg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VLDL ELISA kit uses an anti-VLDL antibody and an VLDL-HRP conjugate in a competitive enzyme immunoassay method. VLDL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VLDL antibody binding site between VLDL from samples and VLDL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VLDL. Since the number of sites is limited, as more sites are occupied by VLDL from the sample, fewer sites are left to bind VLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VLDL concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VLDL and analogues was observed. | |
E02V0006 has been referenced in the below publications：
The Study of Melatonin Regulating Lipid Metabolismin Male Rats.
Effect of melatonin on visceral fat deposition, lipid metabolism and hepatic lipo-metabolic gene expression in male rats.
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