E02V0015 Rat Vitamin A ELISA kit
The Rat Vitamin A ELISA kit can be used to identify samples from the rat species. Vitamin A can also be called Vitamin A, VA.
Specifications of Rat Vitamin A ELISA kit
Product Information | |
Cat. No. | E02V0015 |
Product Name | Rat Vitamin A ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 umol/L |
Sensitivity | 0.1 umol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 umol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 umol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 umol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 umol/L | 1 vial |
STANDARD F (0.5mL) | 10 umol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VA ELISA kit uses an anti-IVA antibody and an IVA-HRP conjugate in a competitive enzyme immunoassay method. IVA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IVA antibody binding site between IVA from samples and IVA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IVA. Since the number of sites is limited, as more sites are occupied by IVA from the sample, fewer sites are left to bind IVA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IVA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Vitamin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VA and analogues was observed. | |
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Summary of the Assay Procedure for Rat Vitamin A ELISA kit
Citations of Rat Vitamin A ELISA kit
E02V0015 has been referenced in the below publications：
Choroid plexus aquaporin 1 and intracranial pressure are increased in obese rats: towards an idiopathic intracranial hypertension model.
