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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Vitamin D3 ELISA kit (E02V0030)

E02V0030 Rat Vitamin D3 ELISA kit

The Rat Vitamin D3 ELISA kit can be used to identify samples from the rat species. Vitamin D3 can also be called vitamin D3, activated 7-dehydrocholesterol, VD3.

Products

Specifications of Rat Vitamin D3 ELISA kit

Product Information

Cat. No.

E02V0030

Product Name

Rat Vitamin D3 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VD3 ELISA kit uses an anti-VD3 antibody and an VD3-HRP conjugate in a competitive enzyme immunoassay method. VD3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VD3 antibody binding site between VD3 from samples and VD3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VD3. Since the number of sites is limited, as more sites are occupied by VD3 from the sample, fewer sites are left to bind VD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VD3 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Vitamin D3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VD3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Vitamin D3 ELISA kit

Summary of the Assay Procedure for Rat Vitamin D3 ELISA kit

Citations of Rat Vitamin D3 ELISA kit

E02V0030 has been referenced in the below publications:

Effect of Icariin on CYP27B1, VDR, CYP24A1, CYP11A1, CYP17A1mRNA expression of adrenal cortex in aging rats induced by D-Galactose.

Effect and significance of ZJXG decoction on the serum levels of CT and VitD3 in femur fracture rats.


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