E02V0030 Rat Vitamin D3 ELISA kit
The Rat Vitamin D3 ELISA kit can be used to identify samples from the rat species. Vitamin D3 can also be called vitamin D3, activated 7-dehydrocholesterol, VD3.
Specifications of Rat Vitamin D3 ELISA kit
Product Information | |
Cat. No. | E02V0030 |
Product Name | Rat Vitamin D3 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VD3 ELISA kit uses an anti-VD3 antibody and an VD3-HRP conjugate in a competitive enzyme immunoassay method. VD3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VD3 antibody binding site between VD3 from samples and VD3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VD3. Since the number of sites is limited, as more sites are occupied by VD3 from the sample, fewer sites are left to bind VD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VD3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Vitamin D3 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VD3 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Vitamin D3 ELISA kit
Citations of Rat Vitamin D3 ELISA kit
E02V0030 has been referenced in the below publications：
Effect of Icariin on CYP27B1, VDR, CYP24A1, CYP11A1, CYP17A1mＲNA expression of adrenal cortex in aging rats induced by D-Galactose.
Effect and significance of ZJXG decoction on the serum levels of CT and VitD3 in femur fracture rats.
