E02V0001 Rat Von Willebrand Factor ELISA kit
The Rat Von Willebrand Factor ELISA kit can be used to identify samples from the rat species. Von Willebrand Factor can also be called F8VWF, VWD, von Willebrand antigen 2, Vwf.
Specifications of Rat Von Willebrand Factor ELISA kit
Product Information | |
Cat. No. | E02V0001 |
Product Name | Rat Von Willebrand Factor ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Vwf ELISA kit uses an anti-Vwf antibody and an Vwf-HRP conjugate in a competitive enzyme immunoassay method. Vwf-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Vwf antibody binding site between Vwf from samples and Vwf-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Vwf. Since the number of sites is limited, as more sites are occupied by Vwf from the sample, fewer sites are left to bind Vwf-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Vwf concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Von Willebrand Factor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Vwf and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Von Willebrand Factor ELISA kit
Citations of Rat Von Willebrand Factor ELISA kit
E02V0001 has been referenced in the below publications：
Expression of vWF in Kidney of diabetic rats and effeet of danhong.
Influence of Endothelial Function and Blood Ｒheology in Diabetic Ｒats by Paeonol and Tanshinol.
