Name: Sheep Calcium Binding Protein D9K ELISA kit (E14C2509)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Sheep Calcium Binding Protein D9K ELISA kit (E14C2509)

E14C2509 Sheep Calcium Binding Protein D9K ELISA kit

Sheep Calcium Binding Protein D9K ELISA kit is suitable for the detection of samples from sheep species. Calcium Binding Protein D9K can also be called CABP1, Calb3, Cabp9k, AU042539, CaBP-D9K, CABPD9k.

Products

Specifications of Sheep Calcium Binding Protein D9K ELISA kit

Product Information
Cat. No.	E14C2509
Product Name	Sheep Calcium Binding Protein D9K ELISA kit
Species	Sheep
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CABPD9k ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CABPD9k antibody and an CABPD9k-HRP conjugate. The assay sample and buffer are incubated together with CABPD9k-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CABPD9k concentration since CABPD9k from samples and CABPD9k-HRP conjugate compete for the anti-CABPD9k antibody binding site. Since the number of sites is limited, as more sites are occupied by CABPD9k from the sample, fewer sites are left to bind CABPD9k-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CABPD9k concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Calcium Binding Protein D9K ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	87-105%
Linearity	Diluent Ratio	Range %
	1:2	84-108
	1:4	81-107
	1:8	80-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CABPD9k and analogues was observed.