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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Fibroblast growth factor 23 ELISA kit (E14F0009)

E14F0009 Sheep Fibroblast growth factor 23 ELISA kit

Sheep Fibroblast growth factor 23 ELISA kit is suitable for the detection of samples from sheep species. Fibroblast growth factor 23 can also be called ADHR, Fibroblast growth factor 23, Fibroblast growth factor 23 N-terminal peptide, Fibroblast growth factor 23 precursor, HPDR2, HYPF, Phosphatonin, PHPTC, FGF 23.

Products

Specifications of Sheep Fibroblast growth factor 23 ELISA kit

Product Information

Cat. No.

E14F0009

Product Name

Sheep Fibroblast growth factor 23 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FGF 23 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FGF 23 antibody and an FGF 23-HRP conjugate. The assay sample and buffer are incubated together with FGF 23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF 23 concentration since FGF 23 from samples and FGF 23-HRP conjugate compete for the anti-FGF 23 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF 23 from the sample, fewer sites are left to bind FGF 23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF 23 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Fibroblast growth factor 23 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FGF 23 and analogues was observed.


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Summary of the Assay Procedure for Sheep Fibroblast growth factor 23 ELISA kit

Summary of the Assay Procedure for Sheep Fibroblast growth factor 23 ELISA kit

Citations of Sheep Fibroblast growth factor 23 ELISA kit

E14F0009 has been referenced in the below publications:

Effects of three blood purification methods on serum fibroblast growth factor‑23 clearance in patients with hyperphosphatemia undergoing maintenance hemodialysis.

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