E14F0009 Sheep Fibroblast growth factor 23 ELISA kit
Sheep Fibroblast growth factor 23 ELISA kit is suitable for the detection of samples from sheep species. Fibroblast growth factor 23 can also be called ADHR, Fibroblast growth factor 23, Fibroblast growth factor 23 N-terminal peptide, Fibroblast growth factor 23 precursor, HPDR2, HYPF, Phosphatonin, PHPTC, FGF 23.
Product Information | |
Cat. No. | E14F0009 |
Product Name | Sheep Fibroblast growth factor 23 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FGF 23 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FGF 23 antibody and an FGF 23-HRP conjugate. The assay sample and buffer are incubated together with FGF 23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF 23 concentration since FGF 23 from samples and FGF 23-HRP conjugate compete for the anti-FGF 23 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF 23 from the sample, fewer sites are left to bind FGF 23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF 23 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FGF 23 and analogues was observed. | |
E14F0009 has been referenced in the below publications:
Effects of three blood purification methods on serum fibroblast growth factor‑23 clearance in patients with hyperphosphatemia undergoing maintenance hemodialysis.
Related Bluegene Biotech Products
BlueGene Biotech News
