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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Follicle Stimulating Hormone ELISA kit (E14F0001)

E14F0001 Sheep Follicle Stimulating Hormone ELISA kit

Sheep Follicle Stimulating Hormone ELISA kit is suitable for the detection of samples from sheep species. Follicle Stimulating Hormone can also be called Follicle Stimulating Hormone, Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin beta chain precursor, Follitropin subunit beta, FSH B, FSH-B, FSH-beta, FSHB, FSHbeta.

Products

Specifications of Sheep Follicle Stimulating Hormone ELISA kit

Product Information

Cat. No.

E14F0001

Product Name

Sheep Follicle Stimulating Hormone ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FSH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FSH antibody and an FSH-HRP conjugate. The assay sample and buffer are incubated together with FSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FSH concentration since FSH from samples and FSH-HRP conjugate compete for the anti-FSH antibody binding site. Since the number of sites is limited, as more sites are occupied by FSH from the sample, fewer sites are left to bind FSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSH concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Follicle Stimulating Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FSH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Follicle Stimulating Hormone ELISA kit

Summary of the Assay Procedure for Sheep Follicle Stimulating Hormone ELISA kit

Citations of Sheep Follicle Stimulating Hormone ELISA kit

E14F0001 has been referenced in the below publications:

Functional verification of Inhibin  β A gene for the sheep breeding traits.

Effects of BMPR-IB Gene on Reproductive Hormone Level in Sheep Peripheral Blood.

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