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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Glutathione Reductase ELISA kit (E14G0238)

E14G0238 Sheep Glutathione Reductase ELISA kit

Sheep Glutathione Reductase ELISA kit is suitable for the detection of samples from Bovine species. Glutathione Reductase can also be called GSR, GR, GSRD, HEL-75, HEL-S-122m, glutathione reductase, glutathione-disulfide reductase.

Products

Specifications of Sheep Glutathione Reductase ELISA kit

Product Information

Cat. No.

E14G0238

Product Name

Sheep Glutathione Reductase ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GR antibody and an GR-HRP conjugate. The assay sample and buffer are incubated together with GR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GR concentration since GR from samples and GR-HRP conjugate compete for the anti-GR antibody binding site. Since the number of sites is limited, as more sites are occupied by GR from the sample, fewer sites are left to bind GR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GR concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Glutathione Reductase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GR and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Glutathione Reductase ELISA kit

Summary of the Assay Procedure for Sheep Glutathione Reductase ELISA kit

Citations of Sheep Glutathione Reductase ELISA kit

E14G0238 has been referenced in the below publications:

Effects of α-Lipoic Acid on Growth Performance,Antioxidant and Immunity of Sheep under Heat Stress.

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