Name: Sheep Gonadotropin Releasing Hormone ELISA kit (E14G0213)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Sheep Gonadotropin Releasing Hormone ELISA kit (E14G0213)

E14G0213 Sheep Gonadotropin Releasing Hormone ELISA kit

Sheep Gonadotropin Releasing Hormone ELISA kit is suitable for the detection of samples from sheep species. Gonadotropin Releasing Hormone can also be called GNRH-1, GnRH associated peptide 1, GNRH1, Gonadotrophin Releasing Hormone 1, GRH, HH12, Luteinizing releasing hormone, Lutenizing Hormome Releasing Hormone, Progonadoliberin 1, Progonadoliberin I, Progonadoliberin1, ProgonadoliberinI, Prolactin Release Inhibiting Factor, Prolctin, GnRH.

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Specifications of Sheep Gonadotropin Releasing Hormone ELISA kit

Product Information
Cat. No.	E14G0213
Product Name	Sheep Gonadotropin Releasing Hormone ELISA kit
Species	Sheep
Product Size	48 Tests / 96 Tests
Concentration	5.0-100 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	5 ng/mL	1 vial
STANDARD C (0.5mL)	10 ng/mL	1 vial
STANDARD D (0.5mL)	25 ng/mL	1 vial
STANDARD E (0.5mL)	50 ng/mL	1 vial
STANDARD F (0.5mL)	100 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

GnRH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GnRH antibody and an GnRH-HRP conjugate. The assay sample and buffer are incubated together with GnRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GnRH concentration since GnRH from samples and GnRH-HRP conjugate compete for the anti-GnRH antibody binding site. Since the number of sites is limited, as more sites are occupied by GnRH from the sample, fewer sites are left to bind GnRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GnRH concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Gonadotropin Releasing Hormone ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	87-105%
Linearity	Diluent Ratio	Range %
	1:2	84-108
	1:4	81-107
	1:8	80-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GnRH and analogues was observed.

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Summary of the Assay Procedure for Sheep Gonadotropin Releasing Hormone ELISA kit

Citations of Sheep Gonadotropin Releasing Hormone ELISA kit

E14G0213 has been referenced in the below publications:

Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle.