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  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Gonadotropin Releasing Hormone ELISA kit (E14G0213)

E14G0213 Sheep Gonadotropin Releasing Hormone ELISA kit

Sheep Gonadotropin Releasing Hormone ELISA kit is suitable for the detection of samples from sheep species. Gonadotropin Releasing Hormone can also be called GNRH-1, GnRH associated peptide 1, GNRH1, Gonadotrophin Releasing Hormone 1, GRH, HH12, Luteinizing releasing hormone, Lutenizing Hormome Releasing Hormone, Progonadoliberin 1, Progonadoliberin I, Progonadoliberin1, ProgonadoliberinI, Prolactin Release Inhibiting Factor, Prolctin, GnRH.

Products

Specifications of Sheep Gonadotropin Releasing Hormone ELISA kit

Product Information

Cat. No.

E14G0213

Product Name

Sheep Gonadotropin Releasing Hormone ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GnRH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GnRH antibody and an GnRH-HRP conjugate. The assay sample and buffer are incubated together with GnRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GnRH concentration since GnRH from samples and GnRH-HRP conjugate compete for the anti-GnRH antibody binding site. Since the number of sites is limited, as more sites are occupied by GnRH from the sample, fewer sites are left to bind GnRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GnRH concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Gonadotropin Releasing Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GnRH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Gonadotropin Releasing Hormone ELISA kit

Summary of the Assay Procedure for Sheep Gonadotropin Releasing Hormone ELISA kit

Citations of Sheep Gonadotropin Releasing Hormone ELISA kit

E14G0213 has been referenced in the below publications:

Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle.

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