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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Growth hormone releasing hormone ELISA kit (E14G0011)

E14G0011 Sheep Growth hormone releasing hormone ELISA kit

Sheep Growth hormone releasing hormone ELISA kit is suitable for the detection of samples from sheep species. Growth hormone releasing hormone can also be called GRF, GHRF, GHRH, GH-RH, HGNC, SRH, SRF, INN, Somatocrinin, Growth-Hormone-Releasing Factor, somatorelin, somatoliberin, somatotropin-releasing hormone, somatotropin-releasing factor.

Products

Specifications of Sheep Growth hormone releasing hormone ELISA kit

Product Information

Cat. No.

E14G0011

Product Name

Sheep Growth hormone releasing hormone ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GHRH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GHRH antibody and an GHRH-HRP conjugate. The assay sample and buffer are incubated together with GHRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHRH concentration since GHRH from samples and GHRH-HRP conjugate compete for the anti-GHRH antibody binding site. Since the number of sites is limited, as more sites are occupied by GHRH from the sample, fewer sites are left to bind GHRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHRH concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Growth hormone releasing hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GHRH and analogues was observed.


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Summary of the Assay Procedure for Sheep Growth hormone releasing hormone ELISA kit

Summary of the Assay Procedure for Sheep Growth hormone releasing hormone ELISA kit

Citations of Sheep Growth hormone releasing hormone ELISA kit

E14G0011 has been referenced in the below publications:

Effects of α-Lipoic Acid on Growth Performance,Antioxidant and Immunity of Sheep under Heat Stress.

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