E14H0022 Sheep Heat Shock Protein 70 ELISA kit
Sheep Heat Shock Protein 70 ELISA kit is suitable for the detection of samples from sheep species. Heat Shock Protein 70 can also be called Hsp70, DnaK, 70 Kilodalton Heat Shock Proteins.
Specifications of Sheep Heat Shock Protein 70 ELISA kit
Product Information | |
Cat. No. | E14H0022 |
Product Name | Sheep Heat Shock Protein 70 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HSP70 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSP70 antibody and an HSP70-HRP conjugate. The assay sample and buffer are incubated together with HSP70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP70 concentration since HSP70 from samples and HSP70-HRP conjugate compete for the anti-HSP70 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP70 from the sample, fewer sites are left to bind HSP70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Heat Shock Protein 70 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HSP70 and analogues was observed. | |
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Summary of the Assay Procedure for Sheep Heat Shock Protein 70 ELISA kit
Citations of Sheep Heat Shock Protein 70 ELISA kit
E14H0022 has been referenced in the below publications:
Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.
