En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Heat Shock Protein 70 ELISA kit (E14H0022)

E14H0022 Sheep Heat Shock Protein 70 ELISA kit

Sheep Heat Shock Protein 70 ELISA kit is suitable for the detection of samples from sheep species. Heat Shock Protein 70 can also be called Hsp70, DnaK, 70 Kilodalton Heat Shock Proteins.

Products

Specifications of Sheep Heat Shock Protein 70 ELISA kit

Product Information

Cat. No.

E14H0022

Product Name

Sheep Heat Shock Protein 70 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HSP70 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HSP70 antibody and an HSP70-HRP conjugate. The assay sample and buffer are incubated together with HSP70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP70 concentration since HSP70 from samples and HSP70-HRP conjugate compete for the anti-HSP70 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP70 from the sample, fewer sites are left to bind HSP70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Heat Shock Protein 70 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HSP70 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Heat Shock Protein 70 ELISA kit

Summary of the Assay Procedure for Sheep Heat Shock Protein 70 ELISA kit

Citations of Sheep Heat Shock Protein 70 ELISA kit

E14H0022 has been referenced in the below publications:

Effects of α-Lipoic Acid on Growth Performance,Antioxidant and Immunity of Sheep under Heat Stress.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.