E14I0009 Sheep Insulin like Growth Factor 1 ELISA kit
Sheep Insulin like Growth Factor 1 ELISA kit is suitable for the detection of samples from sheep species. Insulin like Growth Factor 1 can also be called IBP1, IGF IA, IGF IB, IGFI, IGFIA, Insulin Like Growth Factor 1, Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, MGF, Mechano growth factor, MGF, Somatomedia C, Somatomedin C.
Product Information | |
Cat. No. | E14I0009 |
Product Name | Sheep Insulin like Growth Factor 1 ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IGF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGF1 antibody and an IGF1-HRP conjugate. The assay sample and buffer are incubated together with IGF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF1 concentration since IGF1 from samples and IGF1-HRP conjugate compete for the anti-IGF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF1 from the sample, fewer sites are left to bind IGF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IGF1 and analogues was observed. | |
E14I0009 has been referenced in the below publications:
The Concentration Variation Regularity of GH and IGF-1 in the Peripheral blood of Suffolk Sheeps.
The effect of diets with different protein contents on the IGF 1, GH and growth in sheep.
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