E14L0021 Sheep Luteinizing Hormone ELISA kit
Sheep Luteinizing Hormone ELISA kit is suitable for the detection of samples from sheep species. Luteinizing Hormone can also be called ICSH, Lutropin, Interstitial Cell Stimulating Hormon. LH.
Specifications of Sheep Luteinizing Hormone ELISA kit
Product Information | |
Cat. No. | E14L0021 |
Product Name | Sheep Luteinizing Hormone ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LH. Standards or samples are then added to the microtiter plate wells and LH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LH are added to each well to “sandwich” the LH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Luteinizing Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LH and analogues was observed. | |
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Summary of the Assay Procedure for Sheep Luteinizing Hormone ELISA kit
Citations of Sheep Luteinizing Hormone ELISA kit
E14L0021 has been referenced in the below publications:
Functional verification of Inhibin β A gene for the sheep breeding traits.
Effects of BMPR-IB Gene on Reproductive Hormone Level in Sheep Peripheral Blood.
