E14M0023 Sheep Malondialdehyde ELISA kit
Sheep Malondialdehyde ELISA kit is suitable for the detection of samples from sheep species. Malondialdehyde can also be called MDA, propanedial or malonaldehyde.
Specifications of Sheep Malondialdehyde ELISA kit
Product Information | |
Cat. No. | E14M0023 |
Product Name | Sheep Malondialdehyde ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MDA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MDA antibody and an MDA-HRP conjugate. The assay sample and buffer are incubated together with MDA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MDA concentration since MDA from samples and MDA-HRP conjugate compete for the anti-MDA antibody binding site. Since the number of sites is limited, as more sites are occupied by MDA from the sample, fewer sites are left to bind MDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDA concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Malondialdehyde ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MDA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Sheep Malondialdehyde ELISA kit
Citations of Sheep Malondialdehyde ELISA kit
E14M0023 has been referenced in the below publications:
Effects of α－Lipoic Acid on Growth Performance，Antioxidant and Immunity of Sheep under Heat Stress.
