E14M0035 Sheep Myosin Heavy Chain ELISA kit
Sheep Myosin Heavy Chain ELISA kit is suitable for the detection of samples from sheep species. Myosin Heavy Chain can also be called Alpha MHC, ASD3, CMD1S, CMH1, MPD1, MHC.
Specifications of Sheep Myosin Heavy Chain ELISA kit
Product Information | |
Cat. No. | E14M0035 |
Product Name | Sheep Myosin Heavy Chain ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MHC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MHC antibody and an MHC-HRP conjugate. The assay sample and buffer are incubated together with MHC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MHC concentration since MHC from samples and MHC-HRP conjugate compete for the anti-MHC antibody binding site. Since the number of sites is limited, as more sites are occupied by MHC from the sample, fewer sites are left to bind MHC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MHC concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Myosin Heavy Chain ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MHC and analogues was observed. | |
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Summary of the Assay Procedure for Sheep Myosin Heavy Chain ELISA kit
Citations of Sheep Myosin Heavy Chain ELISA kit
E14M0035 has been referenced in the below publications:
The Comparison of Some Blood Immune Parameters in Sheep.
