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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Non ester Fatty Acid ELISA kit (E14N0063)

E14N0063 Sheep Non ester Fatty Acid ELISA kit

Sheep Non ester Fatty Acid ELISA kit is suitable for the detection of samples from sheep species. Non ester Fatty Acid can also be called FFA, free fatty acid, NEFA.

Products

Specifications of Sheep Non ester Fatty Acid ELISA kit

Product Information

Cat. No.

E14N0063

Product Name

Sheep Non ester Fatty Acid ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 µmol/L

Sensitivity

0.1 µmol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 µmol/L

1 vial

STANDARD B (0.5mL)

0.5 µmol/L

1 vial

STANDARD C (0.5mL)

1.0 µmol/L

1 vial

STANDARD D (0.5mL)

2.5 µmol/L

1 vial

STANDARD E (0.5mL)

5 µmol/L

1 vial

STANDARD F (0.5mL)

10 µmol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NEFA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NEFA antibody and an NEFA-HRP conjugate. The assay sample and buffer are incubated together with NEFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFA concentration since NEFA from samples and NEFA-HRP conjugate compete for the anti-NEFA antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFA from the sample, fewer sites are left to bind NEFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFA concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Non ester Fatty Acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NEFA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Non ester Fatty Acid ELISA kit

Summary of the Assay Procedure for Sheep Non ester Fatty Acid ELISA kit

Citations of Sheep Non ester Fatty Acid ELISA kit

E14N0063 has been referenced in the below publications:

Effects of Eucommia ulmoides Leaves on Sheep Lipid Metabolism and the Mechanism.

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