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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Tryptophan 5 hydroxylase 1 ELISA kit (E14T0726)

E14T0726 Sheep Tryptophan 5 hydroxylase 1 ELISA kit

Sheep Tryptophan 5 hydroxylase 1 ELISA kit is suitable for the detection of samples from sheep species. Tryptophan 5 hydroxylase 1 can also be called TPH1, TPRH, TRPH, tryptophan hydroxylase 1.

Products

Specifications of Sheep Tryptophan 5 hydroxylase 1 ELISA kit

Product Information

Cat. No.

E14T0726

Product Name

Sheep Tryptophan 5 hydroxylase 1 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TPH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TPH1 antibody and an TPH1-HRP conjugate. The assay sample and buffer are incubated together with TPH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TPH1 concentration since TPH1 from samples and TPH1-HRP conjugate compete for the anti-TPH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TPH1 from the sample, fewer sites are left to bind TPH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPH1 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Tryptophan 5 hydroxylase 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TPH1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Tryptophan 5 hydroxylase 1 ELISA kit

Summary of the Assay Procedure for Sheep Tryptophan 5 hydroxylase 1 ELISA kit

Citations of Sheep Tryptophan 5 hydroxylase 1 ELISA kit

E14T0726 has been referenced in the below publications:

Involvement of orexin A in nocturnal melatonin secretion into the cerebrospinal fluid and the blood plasma in seasonal sheep.

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