Application of Rat LH ELISA Kit in Reproductive Research
Role of Luteinizing Hormone
Luteinizing hormone (LH) is secreted by gonadotropic cells in the anterior pituitary and plays a crucial role in regulating the reproductive system. In coordination with follicle-stimulating hormone (FSH), LH promotes follicular development, induces ovulation, and supports corpus luteum formation and maintenance.
In males, LH stimulates Leydig cells in the testes to secrete testosterone, a key hormone for maintaining male reproductive function, muscle growth, and secondary sexual characteristics. Fluctuations in LH levels can impact sperm production and the normal secretion of androgens.
Low LH levels may lead to ovulatory dysfunction, insufficient luteal function, or reduced testosterone secretion in males, potentially affecting fertility. Conversely, elevated LH levels may be associated with endocrine disorders such as polycystic ovary syndrome (PCOS). Quantitative detection of LH is therefore essential for reproductive research and the study of endocrine regulatory mechanisms.
Detection Method of Rat LH ELISA Kit
Sample Suitability
The rat LH ELISA kit is designed for detecting LH levels in rat serum, plasma, and other biological samples. It is widely used in reproductive endocrinology, physiological regulation, and disease research.
Detection Process
When using the rat LH ELISA kit, samples and standards are added to the wells of the microplate. After incubation, a detection antibody is introduced, followed by an enzyme-catalyzed color reaction. The optical density (OD) value is then measured with a microplate reader to determine LH concentration.
Experimental Advantages
High sensitivity for detecting low LH concentrations
Strong specificity without interference from other hormones
Simple operation, suitable for high-throughput experiments
Choosing the Right Rat LH ELISA Kit
The rat LH ELISA kit provides reliable data for reproductive endocrinology research, supporting scientists in exploring LH regulatory mechanisms. BlueGene specializes in the development and production of high-quality ELISA kits, helping researchers worldwide advance reproductive studies.
