Detection Principle and Procedure of Monkey Ubiquitin ELISA
Monkey ubiquitin ELISA is designed for detecting ubiquitin levels in monkey samples and is widely used in studies related to protein degradation and cell signaling. The following sections introduce the detection principle and experimental procedure to help researchers accurately perform the assay.
Detection Principle
Monkey ubiquitin ELISA uses an enzyme-linked immunosorbent assay (ELISA) to detect ubiquitin through specific antigen-antibody binding. The main principles are as follows:
A microplate is pre-coated with a specific antibody to capture ubiquitin in monkey samples.
The sample is added to the microplate, allowing ubiquitin to bind to the coated antibody.
An enzyme-labeled secondary antibody is added, forming an antigen-antibody complex with the bound ubiquitin.
A substrate is introduced, and the enzyme catalyzes a colorimetric reaction, where the color intensity is proportional to the ubiquitin concentration.
The absorbance is measured using a microplate reader to calculate the ubiquitin concentration in the sample.
Experimental Procedure
Monkey ubiquitin ELISA is simple to operate, and the steps are as follows:
Prepare standards, samples, and enzyme reagents according to the instructions.
Add standards or monkey samples to the 96-well plate and incubate at 37°C.
Remove unbound components to ensure detection accuracy.
Add enzyme-labeled antibody, which specifically binds to monkey ubiquitin, and incubate.
Wash to remove unbound antibodies and reduce background interference.
Add the substrate solution; a higher ubiquitin concentration results in a deeper color.
Add the stop solution to prevent further color development.
Analyze data by measuring absorbance at 450 nm with a microplate reader and determine the ubiquitin concentration using a standard curve.
Experimental Considerations
Monkey samples should be free of hemolysis and bacterial contamination to avoid affecting the results.
Residual wash buffer may increase background levels and impact data accuracy.
All reagents should be equilibrated to room temperature to prevent temperature fluctuations from affecting binding reactions.
Ensure accurate dilution of standards to improve the reliability of quantitative detection.
Monkey ubiquitin ELISA, based on antigen-antibody reactions, offers high sensitivity and is suitable for research applications. BlueGene provides monkey ubiquitin ELISA specifically designed for researchers, ensuring stable and reliable experimental data. For more product details, feel free to contact us.
