E11H0013 Bovine Histamine ELISA kit
The Bovine Histamine ELISA kit can be used to identify samples from the bovine species. Histamine can also be called Histamine, Hist, HIS.
Specifications of Bovine Histamine ELISA kit
Product Information | |
Cat. No. | E11H0013 |
Product Name | Bovine Histamine ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HIS ELISA kit uses an anti-HIS antibody and an HIS-HRP conjugate in a competitive enzyme immunoassay method. HIS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HIS antibody binding site between HIS from samples and HIS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HIS. Since the number of sites is limited, as more sites are occupied by HIS from the sample, fewer sites are left to bind HIS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIS concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Histamine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HIS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Histamine ELISA kit
Citations of Bovine Histamine ELISA kit
E11H0013 has been referenced in the below publications：
Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.
Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.
