Bovine Interleukin 12 ELISA kit (E11I0033) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Bovine Interleukin 12 ELISA kit (E11I0033)

E11I0033 Bovine Interleukin 12 ELISA kit

The Bovine Interleukin 12 ELISA kit can be used to identify samples from the bovine species. Interleukin 12 can also be called IL12, CLMF p35, CLMF1, CTL maturation factor (TcMF), Cytotoxic lymphocyte maturation factor 1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL 12 subunit p35, IL12A, Interleukin 12 alpha chain, Interleukin 12 p35, Interleukin 12A, Natural killer cell stimulatory factor 1, CLMF, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, IL12 subunit p35, IL12A, IL12A_HUMAN, Interleukin 12 p35, Interleukin 12A, Interleukin-12 subunit alpha, NFSK, NK cell stimulatory factor chain 1, NKSF1. NK cell stimulatory factor chain 1.

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Specifications of Bovine Interleukin 12 ELISA kit

Product Information
Cat. No.	E11I0033
Product Name	Bovine Interleukin 12 ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL 12. Standards or samples are then added to the microtiter plate wells and IL 12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL 12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL 12 are added to each well to “sandwich” the IL 12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL 12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 12 concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Interleukin 12 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 12 and analogues was observed.

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Summary of the Assay Procedure for Bovine Interleukin 12 ELISA kit

Citations of Bovine Interleukin 12 ELISA kit

E11I0033 has been referenced in the below publications：

Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.