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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human C Reactive Protein ELISA kit (E01C0009)

E01C0009 Human C Reactive Protein ELISA kit

The Human C Reactive Protein ELISA kit can be used to identify samples from the human species. C Reactive Protein can also be called PTX 1, PTX1, C-reactive protein, pentraxin-related, C-Reactive Protein .

Products

Specifications of Human C Reactive Protein ELISA kit

Product Information

Cat. No.

E01C0009

Product Name

Human C Reactive Protein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ug/ml

Sensitivity

1.0 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

5.0 ug/mL

1 vial

STANDARD C (0.5mL)

10 ug/mL

1 vial

STANDARD D (0.5mL)

25 ug/mL

1 vial

STANDARD E (0.5mL)

50 ug/mL

1 vial

STANDARD F (0.5mL)

100 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CRP ELISA kit uses an anti-CRP antibody and an CRP-HRP conjugate in a competitive enzyme immunoassay method. CRP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CRP antibody binding site between CRP from samples and CRP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CRP. Since the number of sites is limited, as more sites are occupied by CRP from the sample, fewer sites are left to bind CRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRP concentration in each sample is interpolated from this standard curve.


Quality Control on Human C Reactive Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Anti-BIP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human C Reactive Protein ELISA kit

Summary of the Assay Procedure for Human C Reactive Protein ELISA kit

Citations of Human C Reactive Protein ELISA kit

E01C0009 has been referenced in the below publications:

Evaluation of Serum Biomarkers in Patients with Sarcoidosis: Can Visfatin Be a New Biomarker for Sarcoidosis?

Effect of different hemodialysis modalities on resistin and nutritional status.

The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.


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