E01C0009 Human C Reactive Protein ELISA kit
The Human C Reactive Protein ELISA kit can be used to identify samples from the human species. C Reactive Protein can also be called PTX 1, PTX1, C-reactive protein, pentraxin-related, C-Reactive Protein .
Specifications of Human C Reactive Protein ELISA kit
Product Information | |
Cat. No. | E01C0009 |
Product Name | Human C Reactive Protein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CRP ELISA kit uses an anti-CRP antibody and an CRP-HRP conjugate in a competitive enzyme immunoassay method. CRP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CRP antibody binding site between CRP from samples and CRP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CRP. Since the number of sites is limited, as more sites are occupied by CRP from the sample, fewer sites are left to bind CRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRP concentration in each sample is interpolated from this standard curve. |
Quality Control on Human C Reactive Protein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Anti-BIP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human C Reactive Protein ELISA kit
Citations of Human C Reactive Protein ELISA kit
E01C0009 has been referenced in the below publications：
Evaluation of Serum Biomarkers in Patients with Sarcoidosis: Can Visfatin Be a New Biomarker for Sarcoidosis?
Effect of different hemodialysis modalities on resistin and nutritional status.
The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.
