E11L0269 Bovine Lymphotactin ELISA kit
The Bovine Lymphotactin ELISA kit can be used to identify samples from the bovine species. Lymphotactin can also be called Lptn, SCYC1, LTN, ATAC, SCM1-A, SCM1a, LPTN, Lymphotactin, Lymphotaxin, Chemokine C-Motif Ligand 1, Small Inducible Cytokine Subfamily C,Member 1, XC chemokine ligand 1, Lptn, LTN.
Product Information | |
Cat. No. | E11L0269 |
Product Name | Bovine Lymphotactin ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Lptn/LTN ELISA kit uses an anti-Lptn/LTN antibody and an Lptn/LTN-HRP conjugate in a competitive enzyme immunoassay method. Lptn/LTN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Lptn/LTN antibody binding site between Lptn/LTN from samples and Lptn/LTN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Lptn/LTN. Since the number of sites is limited, as more sites are occupied by Lptn/LTN from the sample, fewer sites are left to bind Lptn/LTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Lptn/LTN concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Lptn/LTN and analogues was observed. | |
E11L0269 has been referenced in the below publications：
Gene expression profiles in the bovine corpus luteum(CL)during the estrous cycle and pregnancy: Possible roles of chemokines in regulating CL function during pregnancy.
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