E11M0526 Bovine Mannose Binding Lectin A ELISA kit
The Bovine Mannose Binding Lectin A ELISA kit can be used to identify samples from the bovine species. Mannose Binding Lectin A can also be called MBL-A.
Specifications of Bovine Mannose Binding Lectin A ELISA kit
Product Information | |
Cat. No. | E11M0526 |
Product Name | Bovine Mannose Binding Lectin A ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 n/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MBL-A ELISA kit uses an anti-MBL-A antibody and an MBL-A-HRP conjugate in a competitive enzyme immunoassay method. MBL-A-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MBL-A antibody binding site between MBL-A from samples and MBL-A-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MBL-A. Since the number of sites is limited, as more sites are occupied by MBL-A from the sample, fewer sites are left to bind MBL-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBL-A concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Mannose Binding Lectin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MBL-A and analogues was observed. | |
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Summary of the Assay Procedure for Bovine Mannose Binding Lectin A ELISA kit
Citations of Bovine Mannose Binding Lectin A ELISA kit
E11M0526 has been referenced in the below publications：
Mannose-binding lectin 1 haplotypes influence serum MBL-A concentration, complement activity, and milk production traits in Chinese Holstein cattle.
