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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Natural resistance associated macrophage protein 1 ELISA kit (E11N0574)

E11N0574 Bovine Natural resistance associated macrophage protein 1 ELISA kit

The Bovine Natural resistance associated macrophage protein 1 ELISA kit can be used to identify samples from the bovine species. Natural resistance associated macrophage protein 1 can also be called LSH, Natural resistance associated macrophage protein 1, Natural resistance-associated macrophage protein 1, NRAM1_HUMAN, NRAMP 1, NRAMP, PBC, SLC11A1, Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1, solute carrier family 11 (sodium/phosphate symporters) member 1, Solute carrier family 11 member 1.

Products

Specifications of Bovine Albumin ELISA kit_copy32

Product Information

Cat. No.

E11N0574

Product Name

Bovine Natural resistance associated macrophage protein 1 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SLC11A1 ELISA kit uses an anti-SLC11A1 antibody and an SLC11A1-HRP conjugate in a competitive enzyme immunoassay method. SLC11A1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SLC11A1 antibody binding site between SLC11A1 from samples and SLC11A1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SLC11A1. Since the number of sites is limited, as more sites are occupied by SLC11A1 from the sample, fewer sites are left to bind SLC11A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC11A1 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Natural resistance associated macrophage protein 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SLC11A1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Natural resistance associated macrophage protein 1 ELISA kit

Summary of the Assay Procedure for Bovine Natural resistance associated macrophage protein 1 ELISA kit

Citations of Bovine Natural resistance associated macrophage protein 1 ELISA kit

E11N0574 has been referenced in the below publications:

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis.

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