Bovine Natural resistance associated macrophage protein 1 ELISA kit (E11N0574)

BlueGene Biotech Bovine Natural resistance associated macrophage protein 1 ELISA kit (E11N0574)

E11N0574 Bovine Natural resistance associated macrophage protein 1 ELISA kit

The Bovine Natural resistance associated macrophage protein 1 ELISA kit can be used to identify samples from the bovine species. Natural resistance associated macrophage protein 1 can also be called LSH, Natural resistance associated macrophage protein 1, Natural resistance-associated macrophage protein 1, NRAM1_HUMAN, NRAMP 1, NRAMP, PBC, SLC11A1, Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1, solute carrier family 11 (sodium/phosphate symporters) member 1, Solute carrier family 11 member 1.

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Specifications of Bovine Albumin ELISA kit_copy32

Product Information
Cat. No.	E11N0574
Product Name	Bovine Natural resistance associated macrophage protein 1 ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SLC11A1 ELISA kit uses an anti-SLC11A1 antibody and an SLC11A1-HRP conjugate in a competitive enzyme immunoassay method. SLC11A1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SLC11A1 antibody binding site between SLC11A1 from samples and SLC11A1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SLC11A1. Since the number of sites is limited, as more sites are occupied by SLC11A1 from the sample, fewer sites are left to bind SLC11A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC11A1 concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Natural resistance associated macrophage protein 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SLC11A1 and analogues was observed.

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Summary of the Assay Procedure for Bovine Natural resistance associated macrophage protein 1 ELISA kit

Citations of Bovine Natural resistance associated macrophage protein 1 ELISA kit

E11N0574 has been referenced in the below publications：

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis.