E11T0008 Bovine Tumor Necrosis Factor Alpha ELISA kit
The Bovine Tumor Necrosis Factor Alpha ELISA kit can be used to identify samples from the bovine species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF α, TNFα, TNF-α, TNFAlpha, TNF Alpha, TNF-Alpha.
Product Information | |
Cat. No. | E11T0008 |
Product Name | Bovine Tumor Necrosis Factor Alpha ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNFAlpha ELISA kit uses an anti-TNFAlpha antibody and an TNFAlpha-HRP conjugate in a competitive enzyme immunoassay method. TNFAlpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNFAlpha antibody binding site between TNFAlpha from samples and TNFAlpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNFAlpha. Since the number of sites is limited, as more sites are occupied by TNFAlpha from the sample, fewer sites are left to bind TNFAlpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFAlpha concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNFAlpha and analogues was observed. | |
E11T0008 has been referenced in the below publications：
Intervention and Molecular Mechanism of Hydroxy Safflower A on Inflammatory Injury in Bovine Endometrial Epithelial Cells Induced by LPS.
The study of monitoring and diagnostic methods of metritis in dairy cows at different postparturient stages.
SREBP-1c overactivates ROS-mediated hepatic NF-κB inflammatory pathway in dairy cows with fatty liver.
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