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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Matrix metalloproteinase 9 ELISA kit (E08M0329)

E08M0329 Canine Matrix metalloproteinase 9 ELISA kit

Canine Matrix metalloproteinase 9 ELISA kit is suitable for the detection of samples from Canine species. Matrix metalloproteinase 9 can also be called Matrix metalloproteinase-9 precursor, 92 kDa type IV. Collagenase, 92 kDa gelatinase, Gelatinase B, GELB, MMP 9, MMP9, MMP-9.

Products

Specifications of Canine Matrix metalloproteinase 9 ELISA kit

Product Information

Cat. No.

E08M0329

Product Name

Canine Matrix metalloproteinase 9 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MMP 9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP 9. Standards or samples are then added to the microtiter plate wells and MMP 9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP 9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MMP 9 are added to each well to “sandwich” the MMP 9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP 9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 9 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Matrix metalloproteinase 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

86-115%

Linearity

Diluent Ratio

Range %

1:2

84-114

1:4

87-117

1:8

80-112

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MMP 9 and analogues was observed.


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Summary of the Assay Procedure for Canine Matrix metalloproteinase 9 ELISA kit

Summary of the Assay Procedure for Canine Matrix metalloproteinase 9 ELISA kit

Citations of Canine Matrix metalloproteinase 9 ELISA kit

E08M0329 has been referenced in the below publications:

Metoprolol inhibits profibrotic remodeling of epicardial adipose tissue in a canine model of chronic obstructive sleep apnea.

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