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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit (E08T0047)

E08T0047 Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

The Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit can be used to identify samples from the Canine species. Tissue Inhibitor of Metalloproteinases 1 can also be called TIMP 1, Clgi, Collagenase inhibitor, EPA, EPO, Erythroid Potentiating Activity, Fibroblast collagenase inhibitor, HC, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, TIMP, TIMP metallopeptidase inhibitor 1, TIMP1 protein, Tissue Inhibitor of Metalloproteinase 1, Tissue inhibitor of metalloproteinases, Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor.

Products

Specifications of Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Product Information

Cat. No.

E08T0047

Product Name

Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TIMP 1 ELISA kit uses an anti-TIMP 1 antibody and an TIMP 1-HRP conjugate in a competitive enzyme immunoassay method. TIMP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TIMP 1 antibody binding site between TIMP 1 from samples and TIMP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TIMP 1. Since the number of sites is limited, as more sites are occupied by TIMP 1 from the sample, fewer sites are left to bind TIMP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TIMP 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Summary of the Assay Procedure for Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Citations of Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

E08T0047 has been referenced in the below publications:

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.

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