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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Tumor Necrosis Factor Alpha ELISA kit (E08T0008)

E08T0008 Canine Tumor Necrosis Factor Alpha ELISA kit

The Canine Tumor Necrosis Factor Alpha ELISA kit can be used to identify samples from the Canine species. Tumor Necrosis Factor Alpha can also be called TNF-α, TNFα, TNF α, DIF, TNF-A, TNF A, TNFA, TNF-Alpha, TNF Alpha, TNFAlpha, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2.

Products

Specifications of Canine Tumor Necrosis Factor Alpha ELISA kit

Product Information

Cat. No.

E08T0008

Product Name

Canine Tumor Necrosis Factor Alpha ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNFAlpha ELISA kit uses an anti-TNFAlpha antibody and an TNFAlpha-HRP conjugate in a competitive enzyme immunoassay method. TNFAlpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNFAlpha antibody binding site between TNFAlpha from samples and TNFAlpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNFAlpha. Since the number of sites is limited, as more sites are occupied by TNFAlpha from the sample, fewer sites are left to bind TNFAlpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFAlpha concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Tumor Necrosis Factor Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNFAlpha and analogues was observed.


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Summary of the Assay Procedure for Canine Tumor Necrosis Factor Alpha ELISA kit

Summary of the Assay Procedure for Canine Tumor Necrosis Factor Alpha ELISA kit

Citations of Canine Tumor Necrosis Factor Alpha ELISA kit

E08T0008 has been referenced in the below publications:

Metoprolol inhibits profibrotic remodeling of epicardial adipose tissue in a canine model of chronic obstructive sleep apnea.

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