E01E0036 Human Extracellular matrix metalloproteinase lnducer/CD147 ELISA kit
The Human Extracellular matrix metalloproteinase lnducer/CD147 ELISA kit can be used to identify samples from the human species. Extracellular matrix metalloproteinase lnducer/CD147 can also be called BSG, 5F7, CD147, EMMPRIN, M6, OK, TCSF, EMPRIN, SLC7A11, HAb18G, Basigin, cluster of differentiation 147.
Product Information | |
Cat. No. | E01E0036 |
Product Name | Human Extracellular matrix metalloproteinase lnducer/CD147 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| CD147/EMMPRIN ELISA kit uses an anti-CD147/EMMPRIN antibody and an CD147/EMMPRIN-HRP conjugate in a competitive enzyme immunoassay method. CD147/EMMPRIN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CD147/EMMPRIN antibody binding site between CD147/EMMPRIN from samples and CD147/EMMPRIN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CD147/EMMPRIN. Since the number of sites is limited, as more sites are occupied by CD147/EMMPRIN from the sample, fewer sites are left to bind CD147/EMMPRIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD147/EMMPRIN concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CD147/EMMPRIN and analogues was observed. | |
E01E0036 has been referenced in the below publications：
EXPRESSIONS OF EMMPRIN AND ITS LIGAND CYPA IN PERIODONTAL AND PERI-IMPLANTAL GINGIVAL CREVICULAR FLUID.
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