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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Cluster of Differentiation 4 ELISA kit (E12C0004)

E12C0004 Chicken Cluster of Differentiation 4 ELISA kit

Chicken Cluster of Differentiation 4 ELISA kit is suitable for the detection of samples from Chicken species. Cluster of Differentiation 4 can also be called CD4 (L3T4), CD4 antigen (p55), CD4 Antigen, CD4 molecule, CD4 Receptor, CD4+ Lymphocyte deficiency, included, CD4mut.

Products

Specifications of Chicken Cluster of Differentiation 4 ELISA kit

Product Information

Cat. No.

E12C0004

Product Name

Chicken Cluster of Differentiation 4 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD4 antibody and an CD4-HRP conjugate. The assay sample and buffer are incubated together with CD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD4 concentration since CD4 from samples and CD4-HRP conjugate compete for the anti-CD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD4 from the sample, fewer sites are left to bind CD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD4 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Cluster of Differentiation 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CD4 and analogues was observed.


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Summary of the Assay Procedure for Chicken Cluster of Differentiation 4 ELISA kit

Summary of the Assay Procedure for Chicken Cluster of Differentiation 4 ELISA kit

Citations of Chicken Cluster of Differentiation 4 ELISA kit

E12C0004 has been referenced in the below publications:

The Study on Selection of Excellent Bacillus subtilis and Determining its' Growth Performance and Some Biological Indexes.

Screening of Immune Function Related Gene MHC B-LβII and A Certain Purity of Traditional Chinese Medicine Compound Polysaccharides on Immune Regulation in Chicken.

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