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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Granulocyte Colony Stimulating Factor ELISA kit (E12G0112)

E12G0112 Chicken Granulocyte Colony Stimulating Factor ELISA kit

Chicken Granulocyte Colony Stimulating Factor ELISA kit is suitable for the detection of samples from Chicken species. Granulocyte Colony Stimulating Factor can also be called G-CSF, GCSF, CSF3, C17orf33, CSF3OS, colony stimulating factor 3.

Products

Specifications of Chicken Granulocyte Colony Stimulating Factor ELISA kit

Product Information

Cat. No.

E12G0112

Product Name

Chicken Granulocyte Colony Stimulating Factor ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GCSF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GCSF antibody and an GCSF-HRP conjugate. The assay sample and buffer are incubated together with GCSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCSF concentration since GCSF from samples and GCSF-HRP conjugate compete for the anti-GCSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GCSF from the sample, fewer sites are left to bind GCSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCSF concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Granulocyte Colony Stimulating Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GCSF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Granulocyte Colony Stimulating Factor ELISA kit

Summary of the Assay Procedure for Chicken Granulocyte Colony Stimulating Factor ELISA kit

Citations of Chicken Granulocyte Colony Stimulating Factor ELISA kit

E12G0112 has been referenced in the below publications:

Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens.

Effects of compound Chinese herbal medicin al polysaccharides on Immunomodulatory in different MHC B-LBⅡ Genotype Egg-laying Chicks.


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