E12G0016 Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit
Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit is suitable for the detection of samples from Chicken species. Granulocyte Macrophage Colony Stimulating Factor can also be called CSF2, GMCSF, colony stimulating factor 2, CSF, GM CSF.
Product Information | |
Cat. No. | E12G0016 |
Product Name | Chicken Granulocyte Macrophage Colony Stimulating Factor ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GM CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GM CSF antibody and an GM CSF-HRP conjugate. The assay sample and buffer are incubated together with GM CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM CSF concentration since GM CSF from samples and GM CSF-HRP conjugate compete for the anti-GM CSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GM CSF from the sample, fewer sites are left to bind GM CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM CSF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GM CSF and analogues was observed. | |
E12G0016 has been referenced in the below publications:
Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens.
Effects of compound Chinese herbal medicin al polysaccharides on Immunomodulatory in different MHC B-LBⅡ Genotype Egg-laying Chicks.
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