E12I0021 Chicken Immunoglobulin A ELISA kit
Chicken Immunoglobulin A ELISA kit is suitable for the detection of samples from Chicken species. Immunoglobulin A can also be called IgA.
Specifications of Chicken Immunoglobulin A ELISA kit
Product Information | |
Cat. No. | E12I0021 |
Product Name | Chicken Immunoglobulin A ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 mg/mL |
Sensitivity | 0.1 mg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 mg/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 mg/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 mg/mL | 1 vial |
STANDARD E (0.5mL) | 5 mg/mL | 1 vial |
STANDARD F (0.5mL) | 10 mg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IgA antibody and an IgA-HRP conjugate. The assay sample and buffer are incubated together with IgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgA concentration since IgA from samples and IgA-HRP conjugate compete for the anti-IgA antibody binding site. Since the number of sites is limited, as more sites are occupied by IgA from the sample, fewer sites are left to bind IgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgA concentration in each sample is interpolated from this standard curve. |
Quality Control on Chicken Immunoglobulin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgA and analogues was observed. | |
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Summary of the Assay Procedure for Chicken Immunoglobulin A ELISA kit
Citations of Chicken Immunoglobulin A ELISA kit
E12I0021 has been referenced in the below publications:
Effect of dietary oridonin supplementation on growth performance, gut health, and immune response of broilers infected with Salmonella pullorum.
The Study on Selection of Excellent Bacillus subtilis and Determining its' Growth Performance and Some Biological Indexes.
Cytokine and immunoglobulin profiles of Arbor Acres broiler chickens at different stages of physiological development.
