E12I0016 Chicken Immunoglobulin A1 ELISA kit
Chicken Immunoglobulin A1 ELISA kit is suitable for the detection of samples from chicken species. Immunoglobulin A1 can also be called IGA1, Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region.
Product Information | |
Cat. No. | E12I0016 |
Product Name | Chicken Immunoglobulin A1 ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000mg/L |
Sensitivity | 1.0mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 50 mg/L | 1 vial |
STANDARD C (0.5mL) | 100 mg/L | 1 vial |
STANDARD D (0.5mL) | 250 mg/L | 1 vial |
STANDARD E (0.5mL) | 500 mg/L | 1 vial |
STANDARD F (0.5mL) | 1000 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IGA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGA1 antibody and an IGA1-HRP conjugate. The assay sample and buffer are incubated together with IGA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGA1 concentration since IGA1 from samples and IGA1-HRP conjugate compete for the anti-IGA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGA1 from the sample, fewer sites are left to bind IGA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGA1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IGA1 and analogues was observed. | |
E12I0016 has been referenced in the below publications:
Effects of Dietary Supplementation with Glutamine on the Immunity and Intestinal Barrier Gene Expression in Broiler Chickens Infected with Salmonella Enteritidis.
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