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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Immunoglobulin A1 ELISA kit (E12I0016)

E12I0016 Chicken Immunoglobulin A1 ELISA kit

Chicken Immunoglobulin A1 ELISA kit is suitable for the detection of samples from chicken species. Immunoglobulin A1 can also be called IGA1, Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region.

Products

Specifications of Chicken Immunoglobulin A1 ELISA kit

Product Information

Cat. No.

E12I0016

Product Name

Chicken Immunoglobulin A1 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000mg/L

Sensitivity

1.0mg/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 mg/L

1 vial

STANDARD B (0.5mL)

50 mg/L

1 vial

STANDARD C (0.5mL)

100 mg/L

1 vial

STANDARD D (0.5mL)

250 mg/L

1 vial

STANDARD E (0.5mL)

500 mg/L

1 vial

STANDARD F (0.5mL)

1000 mg/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IGA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGA1 antibody and an IGA1-HRP conjugate. The assay sample and buffer are incubated together with IGA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGA1 concentration since IGA1 from samples and IGA1-HRP conjugate compete for the anti-IGA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGA1 from the sample, fewer sites are left to bind IGA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGA1 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Immunoglobulin A1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IGA1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Immunoglobulin A1 ELISA kit

Summary of the Assay Procedure for Chicken Immunoglobulin A1 ELISA kit

Citations of Chicken Immunoglobulin A1 ELISA kit

E12I0016 has been referenced in the below publications:

Effects of Dietary Supplementation with Glutamine on the Immunity and Intestinal Barrier Gene Expression in Broiler Chickens Infected with Salmonella Enteritidis.


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