Name: Chicken Interleukin 6 ELISA kit (E12I0006)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Chicken Interleukin 6 ELISA kit (E12I0006)

E12I0006 Chicken Interleukin 6 ELISA kit

Chicken Interleukin 6 ELISA kit is suitable for the detection of samples from Chicken species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6, IL 6, IL-6.

Products

Specifications of Chicken Interleukin 6 ELISA kit

Product Information
Cat. No.	E12I0006
Product Name	Chicken Interleukin 6 ELISA kit
Species	Chicken
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 6 antibody and an IL 6-HRP conjugate. The assay sample and buffer are incubated together with IL 6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 6 concentration since IL 6 from samples and IL 6-HRP conjugate compete for the anti-IL 6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.

Quality Control on Chicken Interleukin 6 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	91-105%
Linearity	Diluent Ratio	Range %
	1:2	88-106
	1:4	87-108
	1:8	85-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 6 and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Interleukin 6 ELISA kit

Citations of Chicken Interleukin 6 ELISA kit

E12I0006 has been referenced in the below publications:

Screening of Immune Function Related Gene MHC B-LβII and A Certain Purity of Traditional Chinese Medicine Compound Polysaccharides on Immune Regulation in Chicken.

Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.

Effects of Mesobiliverdin IX alpha-Enriched Microalgae Feed on Gut Health and Microbiota of Broilers.