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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Anti Spermiogenesis Antibody ELISA kit (E02A0012)

E02A0012 Rat Anti Spermiogenesis Antibody ELISA kit

Rat Anti spermiogenesis Antibody ELISA kit is suitable for the detection of samples from Rat species. Anti Spermiogenesis Antibody can also be called Anti-sperm autoantibodies, Anti-spermatozoal antibodies, Sperm agglutinating antibodies (SAGA), Sperm immobilizing antibodies (SIA), Sperm-associated antigen (SAA) antibodies. 


Products

Specifications of Rat Anti Spermiogenesis Antibody ELISA kit

Product Information

Cat. No.

E02A0012

Product Name

Rat Anti spermiogenesis Antibody ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ASAb ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ASAb antibody and an ASAb-HRP conjugate. The assay sample and buffer are incubated together with ASAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASAb concentration since ASAb from samples and ASAb-HRP conjugate compete for the anti-ASAb antibody binding site. Since the number of sites is limited, as more sites are occupied by ASAb from the sample, fewer sites are left to bind ASAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASAb concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Anti Spermiogenesis Antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-108%

Linearity

Diluent Ratio

Range %

1:2

90-105

1:4

91-108

1:8

89-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ASAb and analogues was observed.



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Summary of the Assay Procedure for Rat Anti Spermiogenesis Antibody ELISA kit

Summary of the Assay Procedure for Rat Anti Spermiogenesis Antibody ELISA kit

Citations of Rat Anti Spermiogenesis Antibody ELISA kit

E02A0012 has been referenced in the below publications:

The clinical and Experimental Study on Immunity Infertility with Jiajian Guishengye Combined with acupunture.

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