En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit (E07C0209)

E07C0209 Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Porcine soluble Cluster of differentiation 40 ligand ELISA kit is suitable for the detection of samples from porcine species. soluble Cluster of differentiation 40 ligand can also be called CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, Scd40l.

Products

Specifications of Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Product Information

Cat. No.

E07C0209

Product Name

Porcine soluble Cluster of differentiation 40 ligand ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Scd40l ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Scd40l antibody and an Scd40l-HRP conjugate. The assay sample and buffer are incubated together with Scd40l-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Scd40l concentration since Scd40l from samples and Scd40l-HRP conjugate compete for the anti-Scd40l antibody binding site. Since the number of sites is limited, as more sites are occupied by Scd40l from the sample, fewer sites are left to bind Scd40l-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Scd40l concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

93-108%

Linearity

Diluent Ratio

Range %

1:2

95-112

1:4

91-106

1:8

93-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Scd40l and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Summary of the Assay Procedure for Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Citations of Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

E07C0209 has been referenced in the below publications:

Treatment with a histone deacetylase inhibitor, valproic acid, is associated with increased platelet activation in a large animal model of traumatic brain injury and hemorrhagic shock.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.