En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit CD147/EMMPRIN ELISA kit (E04C0234)

E04C0234 Rabbit CD147/EMMPRIN ELISA kit

Rabbit CD147/EMMPRIN ELISA kit is suitable for detecting samples from rabbit species. CD147/EMMPRIN can also be called CD147, TCSF, Emmprin, 5A11 antigen, 5F7,  Basigin, Blood brain barrier HT7 antigen, BSG, CD 147, CD147 antigen, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, M6 leukocyte activation antigen, neurothelin,TCSF, Tumor cell derived collagenase stimulatory factor.

Products

Specifications of Rabbit CD147/EMMPRIN ELISA kit

Product Information

Cat. No.

E04C0234

Product Name

Rabbit CD147/EMMPRIN ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CD147 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CD147 antibody and an CD147-HRP conjugate. The assay sample and buffer are incubated together with CD147-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD147 concentration since CD147 from samples and CD147-HRP conjugate compete for the anti-CD147 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD147 from the sample, fewer sites are left to bind CD147-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD147 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit CD147/EMMPRIN ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CD147 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit CD147/EMMPRIN ELISA kit

Summary of the Assay Procedure for Rabbit CD147/EMMPRIN ELISA kit

Citations of Rabbit CD147/EMMPRIN ELISA kit

E04C0234 has been referenced in the below publications:

The study of stabilizing atherosclerotic vulnerable plaque by gene silencing CD147 using lentiviral-delivered small interference RNA.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.