Name: Goat Interleukin 6 ELISA kit (E06I0006)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Goat Interleukin 6 ELISA kit (E06I0006)

E06I0006 Goat Interleukin 6 ELISA kit

Goat Interleukin 6 ELISA kit is suitable for the detection of samples from goat species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6, IL-6, IL 6, IL6.

Products

Specifications of Goat Interleukin 6 ELISA kit

Product Information
Cat. No.	E06I0006
Product Name	Goat Interleukin 6 ELISA kit
Species	Goat
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 6 antibody and a IL 6-HRP conjugate. The assay sample and buffer are incubated together with IL 6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 6 concentration since IL 6 from samples and IL 6-HRP conjugate compete for the anti- IL 6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.

Quality Control on Goat Interleukin 6 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	87-116.86%
Linearity	Diluent Ratio	Range %
	1:2	92-111
	1:4	88-106
	1:8	85-102
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 6 and analogues was observed.