E05H0013 Guinea Pig Histamine ELISA kit
Guinea Pig Histamine ELISA kit is suitable for the detection of samples from Bovine species. Histamine can also be called Hist, HIS.
Specifications of Guinea Pig Histamine ELISA kit
Product Information | |
Cat. NO. | E05H0013 |
Product Name | Guinea Pig Histamine ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 25 pg/mL | 1 vial |
STANDARD C (0.5mL) | 50 pg/mL | 1 vial |
STANDARD D (0.5mL) | 100 pg/mL | 1 vial |
STANDARD E (0.5mL) | 250 pg/mL | 1 vial |
STANDARD F (0.5mL) | 500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HIS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HIS. Standards or samples are then added to the microtiter plate wells and HIS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HIS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HIS are added to each well to “sandwich” the HIS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HIS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIS concentration in each sample is interpolated from this standard curve. |
Quality Control on Guinea Pig Histamine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 89-108 | |
1:8 | 86-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HIS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Guinea Pig Histamine ELISA kit
Citations of Guinea Pig Histamine ELISA kit
E05H0013 has been referenced in the below publications：
Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Guinea Pigs.
