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BlueGene Biotech Guinea Pig Interferon γ ELISA kit (E05I0345)

E05I0345 Guinea Pig Interferon γ ELISA kit

Guinea Pig Interferon γ ELISA kit is suitable for the detection of samples from Guinea Pig species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG.

Products

Specifications of Guinea Pig Interferon γ ELISA kit

Product Information

Cat. NO.

E05I0345

Product Name

Guinea Pig Interferon γ ELISA kit

Species

Guinea Pig

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IFNγ ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNγ antibody and an IFNγ-HRP conjugate. The assay sample and buffer are incubated together with IFNγ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IFNγ antibody binding site between IFNγ from samples and IFNγ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IFNγ. As more sites are taken up by IFNγ from the sample, fewer sites are left that can bind IFNγ-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the IFNγ concentration in each sample.


Quality Control on Guinea Pig Interferon γ ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-106%

Linearity

Diluent Ratio

Range %

1:2

93-103

1:4

89-108

1:8

86-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IFNγ and analogues was observed.


BlueGene Biotech Product

Summary of the Assay Procedure for Guinea Pig Interferon γ ELISA kit

Summary of the Assay Procedure for Guinea Pig Interferon γ ELISA kit

Citations of Guinea Pig Interferon γ ELISA kit

E05I0345 has been referenced in the below publications:

High-Level Cellular and Humoral Immune Responses in Guinea Pigs Immunized Intradermally with a Heat-Inactivated Varicella-Zoster Virus Vaccine.

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