E05I0024 Guinea Pig Interleukin 5 ELISA kit
Guinea Pig Interleukin 5 ELISA kit is suitable for the detection of samples from Guinea Pig species. Interleukin 5 can also be called B Cell Differentiation Factor I, IL5, IL 5, Colony stimulating factor, Colony Stimulating Factor Eosinophil, EDF, Eosinophil, Eosinophil Differentiation Factor, IL 5, Interleukin 5, B-cell differentiation factor I, IL-5, IL5_RAT, Interleukin-5, T Cell Replacing Factor, T-cell replacing factor, Interleukin5, TRF.
Product Information | |
Cat. NO. | E05I0024 |
Product Name | Guinea Pig Interleukin 5 ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 5 antibody and an IL 5-HRP conjugate. The assay sample and buffer are incubated together with IL 5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 5 antibody binding site between IL 5 from samples and IL 5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 5. As more sites are taken up by IL 5 from the sample, fewer sites are left that can bind IL 5-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. This standard curve is interpolated to determine the IL 5 concentration in each sample. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 89-108 | |
1:8 | 86-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 5 and analogues was observed. | |
E05I0024 has been referenced in the below publications：
“从肝论治, 理气调神”针刺法治疗过敏性鼻炎的实验研究。
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