E10S0252 Horse Soluble endothelium selectin ELISA kit
Horse Soluble endothelium selectin ELISA kit is suitable for the detection of samples from Horse species. Soluble endothelium selectin can also be called sCD62E, sCD62-E, sE-Selectin, sELAM, sELAM1, sESEL, sSEL-E, sE-LAM, sE-LAM1, sE-SEL, sLECAM2, sEndothelial Leukocyte Adhesion Molecule 1, sCD62 Antigen-Like Family Member E, SE selectin.
Product Information | |
Cat. No. | E10S0252 |
Product Name | Horse Soluble endothelium selectin ELISA kit |
Species | Horse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
| Principle of the Assay |
| SE selectin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SE selectin antibody and an SE selectin-HRP conjugate. The assay sample and buffer are incubated together with SE selectin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SE selectin concentration since SE selectin from samples and SE selectin-HRP conjugate compete for the anti-SE selectin antibody binding site. Since the number of sites is limited, as more sites are occupied by SE selectin from the sample, fewer sites are left to bind SE selectin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SE selectin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-109.73% | |
Linearity | Diluent Ratio | Range % |
1:2 | 91-109 | |
1:4 | 89-112 | |
1:8 | 87-115 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SE selectin and analogues was observed. | |
E10S0252 has been referenced in the below publications:
Prospective cohort study evaluating risk factors for the development of pasture-associated laminitis in the United Kingdom.
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